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Description
EasyStep Rat 25-OH-D (25 Hydroxy Vitamin D) ELISA KitThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with Rat 25 OH D antigen, and the Rat 25 OH D standard plate wells that pre coated using protein related techniques are provided separately. Standard Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP conjugated antibody specific to Rat 25 OH D to each microplate
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
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