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Description
Rat SHANK1 (Sh3 and Multiple Ankyrin Repeat Domains Protein 1) ELISA KitThe Rat (SHANK1) Sh3 and Multiple Ankyrin Repeat Domains Protein 1 ELISA Kit measures SH3 and multiple ankyrin repeat domains protein 1 in Rat samples. The plate has been pre coated with Rat Shank1 antibody. Shank1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Shank1 Antibody is added and binds to Shank1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Shank1 antibody.
The Rat (SHANK1) Sh3 and Multiple Ankyrin Repeat Domains Protein 1 ELISA Kit measures SH3 and multiple ankyrin repeat domains protein 1 in Rat samples. The plate has been pre-coated with Rat Shank1 antibody. Shank1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Shank1 Antibody is added and binds to Shank1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Shank1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat Shank1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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