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Description
Mouse F8 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed immediately or stored at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 40 ng/mL). Then dilute to the following concentrations: 40 ng/mL, 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 40ng/mL standard working solution into the first EP tube and mix thoroughly to make a 20ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Coagulation Factor VIII (F8) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Coagulation Factor VIII (F8) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Coagulation Factor VIII ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Factor VII (I FVIII) is an important blood clotting protein, also known as antihemophilic factor (AHF). Factor VIII is encoded by the F8 gene. Defects in this gene cause hemophilia A, a recessive X-linked clotting disorder. Factor VIII is produced in the sinusoidal cells of the liver and the endothelial cells outside the liver, and is found throughout the body. This protein circulates in the blood in an inactive form, bound to another molecule called von Willebrand factor, until an injury occurs that damages a blood vessel. In response to injury, factor VIII becomes activated and dissociates from von Willebrand factor. The active protein (sometimes written as factor VIIIa) interacts with another clotting factor called factor IX. This interaction triggers a cascade of additional chemical reactions that form a blood clot. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.62-40 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma |
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4.3 ★★★★★
Based on 178 reviews
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Product Reviews
★★★★★ 5
I absolutely love the Saltair line of products!
Scent: Sandalwood
I used the body wash at a friend's house and fell in love, especially with the Santal Bloom scent! This body oil is just so amazing and feels so silky and soft. It's especially effective if you use it right out of the shower. It adds a beautiful glow and I even use a little in my hair. It's quite concentrated so a little goes a long way. I have even mixed it into my favorite coconut oil and it mixes beautifully. Don't sleep on this line!
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Reviewed in the United States on February 1, 2026
★★★★★ 5
Smells great
Scent: Golden Hour
Love this, my favorite scent from this line. Very subtle glitter so I still feel age appropriate. It’s an oil soy skin does feel oily for a little bit afterwards until it soaks in. It has lasted me quite awhile so I do feel it’s good value for the money. Have reordered a few times.
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Reviewed in the United States on April 6, 2026
★★★★★ 4
Great Glow and Scent, But Fades Quickly and Runs Out Fast
Scent: Vanilla
The Saltair Shimmering Body Oil in Salt Water Vanilla is a beautiful product.
The only 3 reasons I don’t give it 5 stars are because:
1. The shimmer is not very long-lasting. When I apply it before bed, most of the shimmer effect is completely gone and absorbed by my sheets by the morning. I assume pants and leggings would absorb it in the same way, but I’ve only ever worn shorts after applications during the day, and even then, the shimmer seems to fade after 4–6 hours.
2. I have to apply a lot of oil to achieve the amount of shimmer I desire. It averages about 20–25 pumps per use.
3. The 4-ounce bottle size, combined with needing a lot of product for each application, means I’m going to run out of it pretty quickly.
Here’s why I like this product:
1. The glow: If you’re looking for a subtle glow and a light shimmer effect, this oil is perfect for you; apply it before a night out, while wearing a swimsuit, or when going to the gym in your favorite workout set for a beautiful gleam.
2. The scent: It’s a divine, warm mix of sweet and spicy notes. It smells like vanilla, amber, and toasted marshmallow. It pairs really nicely with tropical, woodsy, sweet, or spicy perfumes—it’s very versatile.
3. Consistency and absorption: The oil absorbs nicely and has a light, runny consistency. Because it’s a dry oil, it isn’t too thick, nor does it leave a sticky feeling on the skin.
4. How my skin feels: I’ve used this oil 4 nights in a row, and every single morning I wake up, my skin feels so soft and smooth, with no residual stickiness or residue left behind.
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Saltair shimmer
Scent: Sandalwood
Nice on the body. Easy to apply. I use it with my lotion for an all over glowy shimmer. It will be very nice in the summer. Nice scent, no irritation.
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Reviewed in the United States on February 15, 2026
★★★★★ 5
Buy it you won’t be disappointed
Scent: Sandalwood
I love it already and I haven’t applied it directly to my full body after a shower as instructed but from what I did apply it’s whatever it says it is . It eats down ok, skin softness, moisturizing , the shimmer is just enough , the scent is soft and smells incredibly great. Smells like a fresh shower lol it’s not thick consistency at all and just the right amount of oiliness to add shine to the body. I can’t wait to use this product fully .
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Reviewed in the United States on July 16, 2025
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