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Description
Rat apo-D ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 ng/mL). Then dilute to the following concentrations: 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200ng/mL standard working solution into the first EP tube and mix thoroughly to make a 100ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Samples, standards, biotin-labeled antibodies, and HRP enzyme conjugates are added sequentially to microwells pre-coated with universal species Apolipoprotein D (apo-D) antigens. After incubation and washing, the substrate TMB is used for color development. TMB is converted to blue under the catalysis of peroxidase (HRP) and to the final yellow under the action of acid. The depth of the color is negatively correlated with the universal species Apolipoprotein D (apo-D) in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Apolipoprotein D ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Apolipoprotein D (apo-D) is a 25 to 30 kDa glycosylated protein and a member of the lipid protein superfamily. As a transporter of several small, hydrophobic molecules, its known biological functions are primarily related to lipid metabolism and neuroprotection. It is a multiligand, multifunctional protein involved in lipid trafficking, food intake, inflammation, antioxidant responses, and the development of various cancer types. A key aspect of its role in lipid metabolism involves the transport of arachidonic acid and the regulation of eicosanoid production and delivery in metabolic tissues. Apo-D expression in metabolic tissues correlates positively and negatively with insulin sensitivity and glucose homeostasis in a tissue-dependent manner. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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★★★★★ 5
Better than most!
Size: 1 add-on extender, Configuration: without eero Plus
I found that this extender works better than most in that it seamlessly boosts the signal without having to switch networks. The set up was super simple and easy, just plug and play basically. Does exactly as advertised, only about a 30% reduction in speed.
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Reviewed in the United States on May 16, 2026
★★★★★ 4
Almost perfect...
Size: 1 router + 2 extenders, Configuration: without eero Plus
I needed to extend my wireless reception for my home office. This was recommended by the cable guy and was easy to install. Instead of just picking up a signal and patching it through, it creates a web in my home and extends the wi-fi not only to my office, but upstairs as well.
It works with my Comcast modem and provides much greater speed and coverage in my home. It's not perfect as I still get occasional freezing when on video calls, but unless I want to move my office to the dining room this will have to do.
I don't know how much this should cost, but I had a little bonus money so I spent it on this upgrade. It seems to be worth it.
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Reviewed in the United States on February 4, 2026
★★★★★ 5
Super Easy Setup & Great Coverage
Size: 1 add-on extender, Configuration: without eero Plus
This was incredibly easy to set up. The app walked me through everything step by step and within just a few minutes the extender was connected and working. I’m not super techy, so I really appreciated how simple and straightforward the process was.
I added this to help improve Wi-Fi coverage in parts of the house that normally had weaker signal, and it made a noticeable difference right away. Everything feels faster and more reliable now, especially when streaming or using multiple devices at once.
Definitely a great option if you want to extend your coverage without dealing with a complicated setup process.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Works with Eero 7 router and easy setup
Size: 1 add-on extender, Configuration: without eero Plus
Fabulous!!
Fortunately I read many reviews and learned a few things before buying this excellent product. I have a recently installed Eero Pro 7, and this works great.
One review I read mentioned it might take a bit of time for your device to recognize use the signal from the extender instead of the router. That was true for me. Give it 30 minutes or so and you will be happy.
Another review talked about having the same Eero router I have, so I knew it would work for me.
And finally, remember this… extenders do not increase speed per se, they improve the strength of the signal. It is improved signal strength that is allowing your device to operate better. Weak signal, slower speed. String signal, faster speed.
Plug it in, open the app, tap to add a device and follow the very, very simple instructions and voila, connected.
Rather than pay the extra $21/mo the IP wanted for me to rent the nodes, for the price of 3 months rental I own the extender.
Excellent product
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Reviewed in the United States on May 1, 2026
★★★★★ 5
Easy-to-add hub to for an existing Eero system
Size: 1 add-on extender, Configuration: without eero Plus
Ok, so I am NOT tech-y at all—but fortunately Eero caters to my ilk!
I installed an Eero wireless "mesh" system when we moved into our home and it came with three hubs to extend the coverage of the wifi throughout the home. That system was very easy to set up and control with an app, even for those without high-tech capacity like myself.
Now, the good thing about a Midcentury home is that it is built insanely well—but the same thing that makes it soundproof and sturdy is also the thing that makes it a little troublesome when it comes to wifi coverage. I made my son a "kid-cave" out of the outdoor shed, which is about 15 feet away from the house. And as good as the Eero was inside the home, it was not able to deliver a consistent signal through the solid concrete walls to the shed.
After much grumping from my 13-year-old gamer, I ordered one more extender hub to solve the issue. It arrived in a timely manner, and I brought it into the shed and plugged it into the wall. Fortunately for me, it was super easy to add this new hub to the existing system. All it took was one click in the app, and in less than a minute, the shed had full, high-speed wifi coverage like the rest of the home.
I cannot recommend Eero enough - it is sleek, small and powerful, and the system is easily extended to suit all your wifi needs.
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Reviewed in the United States on February 24, 2026